Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors

doi:10.1002/advs.202403195

NTSC-IR > 量子频标研究室

	Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors
	Houghton, Matthew C.1,2,3; Toropov, Nikita A.1,2,4; Yu, Deshui 5; Bagby, Stefan 3; Vollmer, Frank 1,2
	2024-07-12
发表期刊	ADVANCED SCIENCE
页码	9
摘要	Optical microcavities, particularly whispering gallery mode (WGM) microcavities enhanced by plasmonic nanorods, are emerging as powerful platforms for single-molecule sensing. However, the impact of optical forces from the plasmonic near field on analyte molecules is inadequately understood. Using a standard optoplasmonic WGM single-molecule sensor to monitor two enzymes, both of which undergo an open-to-closed-to-open conformational transition, the work done on an enzyme by the WGM sensor as atoms of the enzyme move through the electric field gradient of the plasmonic hotspot during conformational change has been quantified. As the work done by the sensor on analyte enzymes can be modulated by varying WGM intensity, the WGM microcavity system can be used to apply free energy penalties to regulate enzyme activity at the single-molecule level. The findings advance the understanding of optical forces in WGM single-molecule sensing, potentially leading to the capability to precisely manipulate enzyme activity at the single-molecule level through tailored optical modulation. Turnover of enzymes bound to optoplasmonic sensors can be detected at the single-molecule level. By modulating the strength of the electric field generated by the plasmonic gold nanoparticle, optoplasmonic sensors may be capable of generating forces that oppose turnover and that could therefore be used to control enzymes at the single molecule level. Created with BioRender.com image
关键词	biosensors enzymes optical forces optoplasmonics peptides and proteins plasmonics
资助者	UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC ; UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC ; UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC ; UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC
DOI	10.1002/advs.202403195
关键词[WOS]	CONFORMATIONAL TRANSITIONS ; KINASE ; POLARIZABILITY ; SHIFT
语种	英语
资助项目	UKRI BBSRC SWBio DTP[BB/T008741/1] ; UKRI BBSRC[BB/Y512977/1] ; UKRI EPSRC[EP/T002875/1]
资助者	UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC ; UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC ; UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC ; UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:001266433300001
出版者	WILEY
引用统计
文献类型	期刊论文
条目标识符	http://210.72.145.45/handle/361003/14494
专题	量子频标研究室
通讯作者	Houghton, Matthew C.; Vollmer, Frank
作者单位	1.Univ Exeter, Living Syst Inst, Exeter EX4 4QD, Devon, England 2.Univ Exeter, Dept Phys & Astron, Exeter EX4 4QD, Devon, England 3.Univ Bath, Dept Life Sci, Bath BA2 7AY, Somerset, England 4.Univ Southampton, Optoelect Res Ctr, Southampton SO17 1BJ, England 5.Chinese Acad Sci, Natl Time Serv Ctr, Xian 710600, Peoples R China
推荐引用方式 GB/T 7714	Houghton, Matthew C.,Toropov, Nikita A.,Yu, Deshui,et al. Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors[J]. ADVANCED SCIENCE,2024:9.
APA	Houghton, Matthew C.,Toropov, Nikita A.,Yu, Deshui,Bagby, Stefan,&Vollmer, Frank.(2024).Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors.ADVANCED SCIENCE,9.
MLA	Houghton, Matthew C.,et al."Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors".ADVANCED SCIENCE (2024):9.